Identification

• Cohort, name
  LiNA
  
  Cohort, abbreviation
  Lifestyle and environmental factors and their Influence on Newborns Allergy risk
  
  Cohort, country
  Germany (Deutschland)
  
  Cohort, website
  https://www.ufz.de/index.php?de=37715
  
  Cohort, facebook-site
  none
  
  Key reference
  mother-child birth cohort, environmental exposure, chemicals, epigenetics, immune system, allergic diseases, obesity
  
  Date of filling in this questionnaire:
  2019-05-06
  
  
• Principal investigator(s), name
  Gunda Herberth
  
  Principal investigator(s), e-mail address
  gunda.herberth@ufz.de
  
  Principal investigator(s), institution
  Helmholtz Centre for Environmental Research
  
  Contact person(s), name
  Stefan Roeder, Gunda Herberth
  
  Contact person(s), e-mail address
  stefan.roeder@ufz.de, gunda.herberth@ufz.de
  
  Contact person(s), institution address
  Helmholtz Centre for Environmental Research
  
  

Basic description

• Main aim of cohort
  The mother-child study LINA (Lifestyle and Environmental factors and their Influence on Newborns Allergy risk) is a birth cohort study designed to investigate the influence of prenatal life style and environmental factors on newborn allergy risk. Six hundred and twenty-two mother-child pairs were recruited from May 2006 to December 2008 in Leipzig, Germany. Maternal blood samples were collected during pregnancy at week 34 of gestation. Venous umbilical cord blood was obtained at delivery. Children and mothers were followed up every year thereafter. Exposure under investigation includes: * chemicals in the environment, * social and life style factors (social economic status, parental education, living conditions, renovation actvities, pet ownership, nutrition, mode of transportation, screen time) Assesed outcomes are: * development of the immune system (IgE, immune parameters, cytokines, chemokines, regulatory T cells) * metabolomics (endogenous metabolites, chemical metabolites) * allergic disorders (atopic dermatitis, hay fever, asthma, allergic sensitisation), * obesity, * behaviour, * respiratory disorders (wheezing, bronchitis, obstructive bronchitis, rhinitis)
  
  Recruited number of:
  629 children / 622 mothers / 0 fathers / 0 grand parents / 0 other family members
  
  Approximate proportion of source population included
  0
  
  Source population
  Region-based
  
  
• Period of enrollment
  Start: 2006-01-01, End: 2008-12-31
  
  Enrollment period
  Pregnancy, week 20 to 34
  
  Enrollment criteria, please specify
  healthy mothers (caucasian), birth weight >=2500 g, gestational age > 36 wk
  
  Exclusion criteria, please specify
  non communicable diseases
  
  Planned age of children at end of follow-up
  Life-long / unrestricted

Questionnaire or registry data and biological samples

• Children
• Mothers
• Fathers

General information	---------- Child age (years) at assessment ----------
	<1	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18
Home address	X	X	X	X	X	X	X	X	X	X	X
School address				X	X	X	X	X	X	X	X

Birth outcomes
Birth weight	X
Birth length	X
Gestational age at birth	X
Apgar score	X
Congenital malformations
Stillbirth (>= 22 weeks)
Child’s sex	X
Head circumference	X
Biparietal diameter

Child exposures	---------- Child age (years) at assessment ----------
	<1	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18
Breast feeding	X	X	X	X
Diet	X				X				X		X
Dietary supplements	X	X	X	X	X	X	X	X	X	X	X
Childcare attendance	X	X	X	X	X	X	X
Active smoking
Passive smoking	X	X	X	X	X	X	X	X	X	X	X
Alcohol consumption
Physical activity							X		X		X
Substance use
Medicine intake
Vaccinations
Psychological distress (e.g. bullying)											X
Air pollution	X	X	X	X	X	X	X		X		X
Access to green spaces	X	X	X	X	X	X	X		X		X
Built environment	X	X	X	X	X	X	X		X		X
Noise	X	X	X	X	X	X	X		X		X
Radiation, electromagnetic fields
Water contamination
Pets	X	X	X	X	X	X	X		X		X
Farm animal exposure
Biomarkers of exposure
Mercury
Lead
Cadmium
Arsenic
Other metals
Polychlorinated biphenyl esters
Dicloro difenil tricloroetano
Hexachlorobenzene	X	X
Brominated flame retardants
Perflouralkyl compounds
Bisphenol A	X
Other bisphenols
Parabens	X
Benzophenones
Triclosan
Phthalates	X
Cotinine	X	X	X	X	X	X	X		X		X

Child development and health	---------- Child age (years) at assessment ----------
	<1	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18
Anthropometry
Weight		X	X	X	X	X	X		X		X
Height		X	X	X	X	X	X		X		X
Head circumference		X	X	X	X	X	X		X		X
Abdominal circumference
Arm circumference
Wrist circumference
Waist circumference
Clinical examinations
Blood pressure
Carotida intima media thickness
Pulse wave velocity
Cardiac ultrasound
Renal ultrasound
Brain MRI
Other MRI
DEXA‐scan results
Skin folds
Bio impedence							X		X		X
Oscilometry
Spirometry							X		X		X
Skin prick test
Bronchial challenge test
Reversibility test
Dental observations												X
Mental and neurological development
Cognitive function
School performance
Language
ADHD									X		X
Autism
Sleep disturbances
Mental health
Respiratory health
Asthma		X	X	X	X	X	X		X		X
Allergy		X	X	X	X	X	X		X		X
Allergic rhinitis		X	X	X	X	X	X		X		X
Eczema		X	X	X	X	X	X		X		X
Respiratory tract infections		X	X	X	X	X	X		X		X
Senses
Vision
Hearing
Musculo-skeletal health
Back pain
Neck pain
Growing pains
Sexual maturation
Tanner stage
Pubertal development stage
Age at menarche
Age at voice change
2D/4D digit ratio
Nocturnal ejaculation
Acne
Other health outcomes
Cancer
Diabetes
Congenital anomalies
Biomarkers of health
Nephrology (e.g. creatinine, albumin)
Thyroid hormones
Inflammatory markers (e.g. C-reactive protein cytokines)	X	X	X	X	X	X	X		X		X
Immunologic markers (e.g. immunoglobulines)	X	X	X	X	X	X	X		X		X
Cortisol
Vitamines and micronutrients	X	X	X
Gonadal axis hormones (e.g. testosterone, estradiol)
Metabolism (e.g. cholesterol, LDL, HDL, insulin, triglycerides)											X
Metabolomics	X	X
DNA methylation	X
Transcriptomics (mRNA, miRNA)	X	X					X
Genome-wide association study (GWAS)

Child biological samples	---------- Child age (years) at sampling ----------
	At birth	0	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18
Whole blood	X		X	X	X	X	X	X		X		X
Serum	X		X	X	X	X	X	X		X		X
Plasma	X		X	X	X	X	X	X		X		X
Buffy coat
Urine						X	X	X		X		X
Hair
Teeth								X	X	X
Nails
Saliva
Purified DNA	X		X	X	X	X	X	X		X		X
Purified RNA	X		X	X	X	X	X	X		X		X
Red blood cells
Umbilical cord blood/tissue
Blood smear
Faeces/meconium												X
Exhaled breathe condensate
Sputum induction
Placenta

General information
	Pre-pregnancy	During pregnancy	<1	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18
Education	X
Income	X			X	X	X	X	X	X		X		X
Employment status	X
Job title
Single parenthood	X
Home address	X
Work address

Maternal characteristics related to the index pregnancy/child
Age at birth	X
Ethnicity	X
Country of birth	X
Planned pregnancy
Time to pregnancy
Fertility treatment
Prior spontaneous abortion (<22w)
Prior births	X
Ultrasound measurements
Doppler measurements
Preeclampsia
Gestational diabetes
Gestational hypertension
Gestational duration
Mode of delivery	X
Prenatal diagnostics
Medical termination of pregnancy

Maternal Exposures	---------- Timing of assessment ----------
	Pre-pregnancy	First trimester	Second trimester	Third trimester	Post pregnancy
Diet		X	X	X
Dietary supplements		X	X	X
Active smoking		X	X	X
Passive smoking		X	X	X
Alcohol consumption		X	X	X
Physical activity
Substance use
Medicine intake		X	X	X
Coffee drinking
Psychological distress (e.g. life-events, health behaviors, perceived stress)		X	X	X
Occupational exposures
Heavy lifts
Work hours		X	X	X
Air pollution		X	X	X
Access to green spaces		X	X	X
Built environment		X	X	X
Noise		X	X	X
Radiation, electromagnetic fields
Water contamination
Binge drinking
Pets		X	X	X
Farm animal exposure
Biomarkers of exposure
Mercury
Lead
Cadmium
Arsenic
Other metals
Polychlorinated biphenyl esters
Dicloro difenil tricloroetano
Hexachlorobenzene
Brominated flame retardants
Perflouralkyl compounds
Bisphenol A		X	X	X
Other bisphenols
Parabens		X	X	X
Benzophenones
Triclosan
Phthalates		X	X	X
Cotinine		X	X	X

Maternal health	---------- Timing of assessment ----------
	Pre-pregnancy	First trimester	Second trimester	Third trimester	Post pregnancy
Anthropometry
Weight
Height
Waist circumference
Clinical examinations
Blood pressure
Carotida intima media thickness
Pulse wave velocity
DEXA scans
Bioimpedence
Mental and neurological development
Mental health
Cognitive function (e.g. intelligence quotient)
Respiratory health
Asthma		X	X	X
Allergy		X	X	X
Musculo‐skeletal disease
Back pain
Neck pain
Arthritis
Other health outcomes
Cancer
Diabetes
Cardio‐vascular disease
Family history of chronic disease
Infectious disease
Fever
Autoimmune disease
Urinary symptoms
Sexual problems
Sleep disturbance
Biomarkers of health
Nephrology (e.g. creatinine, albumin)
Thyroid hormones
Inflammatory markers (e.g. C-reactive protein cytokines)				X
Immunologic markers (e.g. immunoglobulines)				X
Cortisol
Vitamines and micronutrients		X	X	X
Gonadal axis hormones (e.g. testosterone, estradiol)
Metabolism (e.g. cholesterol, LDL, HDL, insulin, triglycerides)
Metabolomics				X
DNA methylation				X
Transcriptomics (mRNA, miRNA)				X
Genome-wide association study (GWAS)

Maternal biological samples	---------- Timing of sampling ----------
	Pre-pregnancy	First trimester	Second trimester	Third trimester	Post pregnancy
Breast milk
Whole blood				X
Serum				X
Plasma				X
Buffy coat
Urine				X
Hair
Nails
Saliva
Purified DNA				X
Purified RNA				X
Red blood cells
Faeces

General information
	Pre-pregnancy	During pregnancy	<1	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18
Education
Income
Employment status
Job title
Single parenthood
Home address
Work address

Paternal characteristics related to the index pregnancy/child
Fertility treatment
Age at child's birth
Ethnicity
Country of birth

Paternal exposures	---------- Timing of assessment ----------
	Pre-pregnancy	During pregnancy	Post pregnancy
Diet
Dietary supplements
Active smoking
Passive smoking
Alcohol consumption
Physical activity
Substance use
Medicine intake
Coffee drinking
Psychological distress (e.g. life-events, health behaviors, perceived stress)
Occupational exposures
Heavy lifts
Work hours
Air pollution
Access to green spaces
Built environment
Noise
Radiation, electromagnetic fields
Water contamination
Binge drinking
Pets
Farm animal exposure
Biomarkers of exposure
Mercury
Lead
Cadmium
Arsenic
Other metals
Polychlorinated biphenyl esters
Dicloro difenil tricloroetano
Hexachlorobenzene
Brominated flame retardants
Perflouralkyl compounds
Bisphenol A
Other bisphenols
Parabens
Benzophenones
Triclosan
Phthalates
Cotinine

Paternal health	---------- Timing of assessment ----------
	Pre-pregnancy	During pregnancy	Post pregnancy
Anthropometry
Weight
Height
Waist circumference
Clinical examinations
Blood pressure
Carotida intima media thickness
Pulse wave velocity
DEXA scans
Bio impedence
Mental and neurological development
Mental health
Cognitive function (e.g. intelligence quotient)
Respiratory health
Asthma	X	X
Allergy	X	X
Musculo‐skeletal disease
Back pain
Neck pain
Arthritis
Other health outcomes
Cancer
Diabetes
Cardio‐vascular disease
Family history of chronic disease
Infectious disease
Fever
Autoimmune disease
Urinary symptoms
Sexual problems
Sleep disturbance
Biomarkers of health
Nephrology (e.g. creatinine, albumin)
Thyroid hormones
Inflammatory markers (e.g. C-reactive protein cytokines)
Immunologic markers (e.g. immunoglobulines)
Cortisol
Vitamines and micronutrients
Gonadal axis hormones (e.g. testosterone, estradiol)
Metabolism (e.g. cholesterol, LDL, HDL, insulin, triglycerides)
Metabolomics
DNA methylation
Transcriptomics (mRNA, miRNA)
Genome-wide association study (GWAS)

Paternal biological samples	---------- Timing of sampling ----------
	Pre-pregnancy	During pregnancy	Post pregnancy
Semen
Whole blood
Serum
Plasma
Buffy coat
Urine
Hair
Nails
Saliva
Purified DNA
Purified RNA
Red blood cells
Faeces

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