Identification

• Cohort, name
  FLemish Environment and Health Study 3 Reference Newborns
  
  Cohort, abbreviation
  FLEHS III
  
  Cohort, country
  Belgium (Belgi
  
  Cohort, website
  http://www.milieu-en-gezondheid.be/en/homepage-eng
  
  Date of filling in this questionnaire:
  0000-00-00
  
  
• Principal investigator(s), name
  Prof. dr. Greet Schoeters
  
  Principal investigator(s), e-mail address
  greet.schoeters@vito.be
  
  Principal investigator(s), institution
  VITO, Boeretang 200, 2400 Mol, Belgium
  
  Contact person(s), name
  Prof. dr. Greet Schoeters
  
  Contact person(s), e-mail address
  Prof. dr. Greet Schoeters
  
  Contact person(s), institution address
  VITO, Boeretang 200, 2400 Mol, Belgium
  
  

Basic description

• Main aim of cohort
  To identify determinants of exposure. To investigate whether pollutants or mixtures of pollutants in the general population are associated with molecular and/or clinical effects. To explore time trends for pollutants measured in FLEHS I, II & III. To establish reference values for the general Flemish population for a large set of biomarkers of exposure. Simultaneously, relevant health effects are measured in cord blood/hair or assessed through questionnaires.
  
  Recruited number of:
  281 children / 281 mothers / 0 fathers / 0 grand parents / 0 other family members
  
  Approximate proportion of source population included
  0
  
  Source population
  Region-based
  
  
• Period of enrollment
  Start: 2013-11-04, End: 2014-11-07
  
  Enrollment period
  At birth  to
  
  Enrollment criteria, please specify
  Inclusion criteria mothers: - They should live in Flanders for at least 10 years - They should provide consent: this means the participants are willing to provide a sample of cord blood, blood and hair and are willing to fill in an extended questionnaire the week after they give birth and fill in a questionanire about the neurological development of their child for 2 years. They should be able to fill in the Dutch questionnaire (with questions about pregancy/health/social parameters/living environment/lifestyle/smoking/ alcohol/ nutrition and perception about environment and health).
  
  Exclusion criteria, please specify
  /
  
  Planned age of children at end of follow-up
  

Questionnaire or registry data and biological samples

• Children
• Mothers
• Fathers

General information	---------- Child age (years) at assessment ----------
	<1	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	>18
Home address
School address

Birth outcomes
Birth weight	X
Birth length	X
Gestational age at birth	X
Apgar score
Congenital malformations	X
Stillbirth (>= 22 weeks)
Child’s sex	X
Head circumference	X
Biparietal diameter

Child exposures	---------- Child age (years) at assessment ----------
	<1	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	>18
Breast feeding
Diet
Dietary supplements
Childcare attendance
Active smoking
Passive smoking
Alcohol consumption
Physical activity
Substance use
Medicine intake
Vaccinations
Psychological distress (e.g. bullying)
Air pollution
Access to green spaces
Built environment
Noise
Radiation, electromagnetic fields
Water contamination
Pets
Farm animal exposure
Biomarkers of exposure
Mercury
Lead	X
Cadmium	X
Arsenic	X
Other metals	X
Polychlorinated biphenyl esters	X
Dicloro difenil tricloroetano	X
Hexachlorobenzene	X
Brominated flame retardants
Perflouralkyl compounds	X
Bisphenol A
Other bisphenols
Parabens
Benzophenones
Triclosan
Phthalates
Cotinine

Child development and health	---------- Child age (years) at assessment ----------
	<1	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	>18
Anthropometry
Weight
Height
Head circumference
Abdominal circumference
Arm circumference
Wrist circumference
Waist circumference
Clinical examinations
Blood pressure
Carotida intima media thickness
Pulse wave velocity
Cardiac ultrasound
Renal ultrasound
Brain MRI
Other MRI
DEXA‐scan results
Skin folds
Bio impedence
Oscilometry
Spirometry
Skin prick test
Bronchial challenge test
Reversibility test
Dental observations
Mental and neurological development
Cognitive function
School performance
Language
ADHD
Autism
Sleep disturbances
Mental health
Respiratory health
Asthma
Allergy
Allergic rhinitis
Eczema
Respiratory tract infections
Senses
Vision
Hearing
Musculo-skeletal health
Back pain
Neck pain
Growing pains
Sexual maturation
Tanner stage
Pubertal development stage
Age at menarche
Age at voice change
2D/4D digit ratio
Nocturnal ejaculation
Acne
Other health outcomes
Cancer
Diabetes
Congenital anomalies
Biomarkers of health
Nephrology (e.g. creatinine, albumin)
Thyroid hormones	X
Inflammatory markers (e.g. C-reactive protein cytokines)
Immunologic markers (e.g. immunoglobulines)
Cortisol
Vitamines and micronutrients
Gonadal axis hormones (e.g. testosterone, estradiol)	X
Metabolism (e.g. cholesterol, LDL, HDL, insulin, triglycerides)	X
Metabolomics
DNA methylation
Transcriptomics (mRNA, miRNA)
Genome-wide association study (GWAS)

Child biological samples	---------- Child age (years) at sampling ----------
	At birth	0	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	>18
Whole blood
Serum
Plasma
Buffy coat
Urine
Hair
Teeth
Nails
Saliva
Purified DNA
Purified RNA
Red blood cells
Umbilical cord blood/tissue	X
Blood smear
Faeces/meconium
Exhaled breathe condensate
Sputum induction
Placenta

General information
	Pre-pregnancy	During pregnancy	<1	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18
Education		X
Income		X
Employment status		X
Job title		X
Single parenthood		X
Home address
Work address

Maternal characteristics related to the index pregnancy/child
Age at birth	X
Ethnicity
Country of birth	X
Planned pregnancy
Time to pregnancy	X
Fertility treatment	X
Prior spontaneous abortion (<22w)	X
Prior births	X
Ultrasound measurements
Doppler measurements
Preeclampsia
Gestational diabetes
Gestational hypertension
Gestational duration
Mode of delivery	X
Prenatal diagnostics
Medical termination of pregnancy

Maternal Exposures	---------- Timing of assessment ----------
	Pre-pregnancy	First trimester	Second trimester	Third trimester	Post pregnancy
Diet	X	X	X	X	X
Dietary supplements	X	X	X	X
Active smoking	X	X	X	X
Passive smoking	X
Alcohol consumption	X	X	X	X
Physical activity	X
Substance use	X	X	X	X
Medicine intake
Coffee drinking	X	X	X	X	X
Psychological distress (e.g. life-events, health behaviors, perceived stress)		X	X	X
Occupational exposures	X
Heavy lifts
Work hours
Air pollution
Access to green spaces
Built environment
Noise
Radiation, electromagnetic fields
Water contamination
Binge drinking
Pets
Farm animal exposure
Biomarkers of exposure
Mercury
Lead
Cadmium
Arsenic
Other metals
Polychlorinated biphenyl esters
Dicloro difenil tricloroetano
Hexachlorobenzene
Brominated flame retardants
Perflouralkyl compounds
Bisphenol A
Other bisphenols
Parabens
Benzophenones
Triclosan
Phthalates
Cotinine

Maternal health	---------- Timing of assessment ----------
	Pre-pregnancy	First trimester	Second trimester	Third trimester	Post pregnancy
Anthropometry
Weight	X
Height	X
Waist circumference
Clinical examinations
Blood pressure
Carotida intima media thickness
Pulse wave velocity
DEXA scans
Bioimpedence
Mental and neurological development
Mental health
Cognitive function (e.g. intelligence quotient)
Respiratory health
Asthma					X
Allergy					X
Musculo‐skeletal disease
Back pain
Neck pain
Arthritis
Other health outcomes
Cancer
Diabetes
Cardio‐vascular disease
Family history of chronic disease
Infectious disease		X	X	X
Fever
Autoimmune disease
Urinary symptoms
Sexual problems
Sleep disturbance
Biomarkers of health
Nephrology (e.g. creatinine, albumin)
Thyroid hormones
Inflammatory markers (e.g. C-reactive protein cytokines)
Immunologic markers (e.g. immunoglobulines)
Cortisol
Vitamines and micronutrients
Gonadal axis hormones (e.g. testosterone, estradiol)
Metabolism (e.g. cholesterol, LDL, HDL, insulin, triglycerides)
Metabolomics
DNA methylation
Transcriptomics (mRNA, miRNA)
Genome-wide association study (GWAS)

Maternal biological samples	---------- Timing of sampling ----------
	Pre-pregnancy	First trimester	Second trimester	Third trimester	Post pregnancy
Breast milk
Whole blood					X
Serum
Plasma
Buffy coat
Urine
Hair					X
Nails
Saliva
Purified DNA
Purified RNA
Red blood cells
Faeces

General information
	Pre-pregnancy	During pregnancy	<1	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18
Education
Income
Employment status
Job title
Single parenthood
Home address
Work address

Paternal characteristics related to the index pregnancy/child
Fertility treatment
Age at child's birth
Ethnicity
Country of birth

Paternal exposures	---------- Timing of assessment ----------
	Pre-pregnancy	During pregnancy	Post pregnancy
Diet
Dietary supplements
Active smoking
Passive smoking
Alcohol consumption
Physical activity
Substance use
Medicine intake
Coffee drinking
Psychological distress (e.g. life-events, health behaviors, perceived stress)
Occupational exposures
Heavy lifts
Work hours
Air pollution
Access to green spaces
Built environment
Noise
Radiation, electromagnetic fields
Water contamination
Binge drinking
Pets
Farm animal exposure
Biomarkers of exposure
Mercury
Lead
Cadmium
Arsenic
Other metals
Polychlorinated biphenyl esters
Dicloro difenil tricloroetano
Hexachlorobenzene
Brominated flame retardants
Perflouralkyl compounds
Bisphenol A
Other bisphenols
Parabens
Benzophenones
Triclosan
Phthalates
Cotinine

Paternal health	---------- Timing of assessment ----------
	Pre-pregnancy	During pregnancy	Post pregnancy
Anthropometry
Weight
Height
Waist circumference
Clinical examinations
Blood pressure
Carotida intima media thickness
Pulse wave velocity
DEXA scans
Bio impedence
Mental and neurological development
Mental health
Cognitive function (e.g. intelligence quotient)
Respiratory health
Asthma
Allergy
Musculo‐skeletal disease
Back pain
Neck pain
Arthritis
Other health outcomes
Cancer
Diabetes
Cardio‐vascular disease
Family history of chronic disease
Infectious disease
Fever
Autoimmune disease
Urinary symptoms
Sexual problems
Sleep disturbance
Biomarkers of health
Nephrology (e.g. creatinine, albumin)
Thyroid hormones
Inflammatory markers (e.g. C-reactive protein cytokines)
Immunologic markers (e.g. immunoglobulines)
Cortisol
Vitamines and micronutrients
Gonadal axis hormones (e.g. testosterone, estradiol)
Metabolism (e.g. cholesterol, LDL, HDL, insulin, triglycerides)
Metabolomics
DNA methylation
Transcriptomics (mRNA, miRNA)
Genome-wide association study (GWAS)

Paternal biological samples	---------- Timing of sampling ----------
	Pre-pregnancy	During pregnancy	Post pregnancy
Semen
Whole blood
Serum
Plasma
Buffy coat
Urine
Hair
Nails
Saliva
Purified DNA
Purified RNA
Red blood cells
Faeces

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Comments and ideas