Identification

• Cohort, name
  ShengJing Birth Cohort
  
  Cohort, abbreviation
  SJBC
  
  Cohort, country
  China (中国)
  
  Date of filling in this questionnaire:
  2018-03-28
  
  
• Principal investigator(s), name
  Prof Dr Zhengwei Yuan
  
  Principal investigator(s), e-mail address
  yuanzw@sj-hospital.org
  
  Principal investigator(s), institution
  Shengjing Hospital, China Medical University
  
  Contact person(s), name
  Jia Xue
  
  Contact person(s), e-mail address
  459125186@qq.com
  
  Contact person(s), institution address
  Shengjing Hospital, China Medical University
  
  

Basic description

• Main aim of cohort
  To examine the effects of genetic, nutritional, environmental, and social factors on maternal, child, and adolescent health outcomes. The research results will help promote and improve the health status of the next generation of children and their families in China and may even be extended to other countries or regions.
  
  Recruited number of:
  20000 children / 20000 mothers / 20000 fathers / 0 grand parents / 0 other family members
  
  Approximate proportion of source population included
  0
  
  Source population
  Region-based
  
  
• Period of enrollment
  Start: 2017-05-10, End: 0000-00-00
  
  Enrollment period
  Pregnancy, week 1 to 20
  
  Enrollment criteria, please specify
  meeting the following three conditions: 1, pregnant women 20 weeks before gestation 2, planning to give birth at shengjing hospital 3, residing in Northeast China and no relocation plan within 3 years of their children
  
  Exclusion criteria, please specify
  Not familiar with Chinese language in word and writing.
  
  Planned age of children at end of follow-up
  18 years

Questionnaire or registry data and biological samples

• Children
• Mothers
• Fathers

General information	---------- Child age (years) at assessment ----------
	<1	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	>18
Home address	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
School address	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X

Birth outcomes
Birth weight	X
Birth length	X
Gestational age at birth	X
Apgar score
Congenital malformations	X
Stillbirth (>= 22 weeks)	X
Child’s sex	X
Head circumference	X
Biparietal diameter	X

Child exposures	---------- Child age (years) at assessment ----------
	<1	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	>18
Breast feeding	X	X	X	X
Diet	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Dietary supplements	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Childcare attendance	X	X	X	X	X	X	X
Active smoking											X	X	X	X	X	X	X	X	X
Passive smoking	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Alcohol consumption											X	X	X	X	X	X	X	X	X
Physical activity	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Substance use											X	X	X	X	X	X	X	X	X
Medicine intake	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Vaccinations	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Psychological distress (e.g. bullying)	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Air pollution	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Access to green spaces	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Built environment	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Noise	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Radiation, electromagnetic fields	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Water contamination	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Pets	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Farm animal exposure	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Biomarkers of exposure
Mercury	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Lead	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Cadmium	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Arsenic	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Other metals	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Polychlorinated biphenyl esters	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Dicloro difenil tricloroetano	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Hexachlorobenzene	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Brominated flame retardants	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Perflouralkyl compounds	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Bisphenol A	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Other bisphenols	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Parabens	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Benzophenones	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Triclosan	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Phthalates	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Cotinine	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X

Child development and health	---------- Child age (years) at assessment ----------
	<1	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	>18
Anthropometry
Weight	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Height	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Head circumference	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Abdominal circumference	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Arm circumference	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Wrist circumference	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Waist circumference	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Clinical examinations
Blood pressure	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Carotida intima media thickness	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Pulse wave velocity	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Cardiac ultrasound	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Renal ultrasound	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Brain MRI	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Other MRI	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
DEXA‐scan results	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Skin folds	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Bio impedence	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Oscilometry	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Spirometry	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Skin prick test	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Bronchial challenge test	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Reversibility test	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Dental observations	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Mental and neurological development
Cognitive function	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
School performance					X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Language		X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
ADHD	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Autism	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Sleep disturbances	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Mental health	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Respiratory health
Asthma	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Allergy	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Allergic rhinitis	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Eczema	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Respiratory tract infections	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Senses
Vision	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Hearing	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Musculo-skeletal health
Back pain	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Neck pain	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Growing pains	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Sexual maturation
Tanner stage	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Pubertal development stage	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Age at menarche										X	X	X	X	X	X	X	X	X	X
Age at voice change	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
2D/4D digit ratio	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Nocturnal ejaculation	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Acne	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Other health outcomes
Cancer	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Diabetes	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Congenital anomalies	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Biomarkers of health
Nephrology (e.g. creatinine, albumin)	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Thyroid hormones	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Inflammatory markers (e.g. C-reactive protein cytokines)	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Immunologic markers (e.g. immunoglobulines)	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Cortisol	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Vitamines and micronutrients	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Gonadal axis hormones (e.g. testosterone, estradiol)	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Metabolism (e.g. cholesterol, LDL, HDL, insulin, triglycerides)	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Metabolomics	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
DNA methylation	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Transcriptomics (mRNA, miRNA)	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Genome-wide association study (GWAS)	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X

Child biological samples	---------- Child age (years) at sampling ----------
	At birth	0	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	>18
Whole blood	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Serum	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Plasma	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Buffy coat	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Urine	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Hair	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Teeth		X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Nails	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Saliva	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Purified DNA	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Purified RNA	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Red blood cells	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Umbilical cord blood/tissue	X
Blood smear	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Faeces/meconium	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Exhaled breathe condensate	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Sputum induction	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Placenta	X

General information
	Pre-pregnancy	During pregnancy	<1	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18
Education	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Income	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Employment status	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Job title	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Single parenthood	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Home address	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Work address	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X

Maternal characteristics related to the index pregnancy/child
Age at birth	X
Ethnicity	X
Country of birth	X
Planned pregnancy	X
Time to pregnancy	X
Fertility treatment	X
Prior spontaneous abortion (<22w)	X
Prior births	X
Ultrasound measurements	X
Doppler measurements	X
Preeclampsia	X
Gestational diabetes	X
Gestational hypertension	X
Gestational duration	X
Mode of delivery	X
Prenatal diagnostics	X
Medical termination of pregnancy	X

Maternal Exposures	---------- Timing of assessment ----------
	Pre-pregnancy	First trimester	Second trimester	Third trimester	Post pregnancy
Diet	X	X	X	X	X
Dietary supplements	X	X	X	X	X
Active smoking	X	X	X	X	X
Passive smoking	X	X	X	X	X
Alcohol consumption	X	X	X	X	X
Physical activity	X	X	X	X	X
Substance use	X	X	X	X	X
Medicine intake	X	X	X	X	X
Coffee drinking	X	X	X	X	X
Psychological distress (e.g. life-events, health behaviors, perceived stress)	X	X	X	X	X
Occupational exposures	X	X	X	X	X
Heavy lifts	X	X	X	X	X
Work hours	X	X	X	X	X
Air pollution	X	X	X	X	X
Access to green spaces	X	X	X	X	X
Built environment	X	X	X	X	X
Noise	X	X	X	X	X
Radiation, electromagnetic fields	X	X	X	X	X
Water contamination	X	X	X	X	X
Binge drinking	X	X	X	X	X
Pets	X	X	X	X	X
Farm animal exposure	X	X	X	X	X
Biomarkers of exposure
Mercury	X	X	X	X	X
Lead	X	X	X	X	X
Cadmium	X	X	X	X	X
Arsenic	X	X	X	X	X
Other metals	X	X	X	X	X
Polychlorinated biphenyl esters	X	X	X	X	X
Dicloro difenil tricloroetano	X	X	X	X	X
Hexachlorobenzene	X	X	X	X	X
Brominated flame retardants	X	X	X	X	X
Perflouralkyl compounds	X	X	X	X	X
Bisphenol A	X	X	X	X	X
Other bisphenols	X	X	X	X	X
Parabens	X	X	X	X	X
Benzophenones	X	X	X	X	X
Triclosan	X	X	X	X	X
Phthalates	X	X	X	X	X
Cotinine	X	X	X	X	X

Maternal health	---------- Timing of assessment ----------
	Pre-pregnancy	First trimester	Second trimester	Third trimester	Post pregnancy
Anthropometry
Weight	X	X	X	X	X
Height	X	X	X	X	X
Waist circumference	X	X	X	X	X
Clinical examinations
Blood pressure	X	X	X	X	X
Carotida intima media thickness	X	X	X	X	X
Pulse wave velocity	X	X	X	X	X
DEXA scans					X
Bioimpedence					X
Mental and neurological development
Mental health	X	X	X	X	X
Cognitive function (e.g. intelligence quotient)	X	X	X	X	X
Respiratory health
Asthma	X	X	X	X	X
Allergy	X	X	X	X	X
Musculo‐skeletal disease
Back pain	X	X	X	X	X
Neck pain	X	X	X	X	X
Arthritis	X	X	X	X	X
Other health outcomes
Cancer	X	X	X	X	X
Diabetes	X	X	X	X	X
Cardio‐vascular disease	X	X	X	X	X
Family history of chronic disease	X	X	X	X	X
Infectious disease	X	X	X	X	X
Fever	X	X	X	X	X
Autoimmune disease	X	X	X	X	X
Urinary symptoms	X	X	X	X	X
Sexual problems	X	X	X	X	X
Sleep disturbance	X	X	X	X	X
Biomarkers of health
Nephrology (e.g. creatinine, albumin)	X	X	X	X	X
Thyroid hormones	X	X	X	X	X
Inflammatory markers (e.g. C-reactive protein cytokines)	X	X	X	X	X
Immunologic markers (e.g. immunoglobulines)	X	X	X	X	X
Cortisol	X	X	X	X	X
Vitamines and micronutrients	X	X	X	X	X
Gonadal axis hormones (e.g. testosterone, estradiol)	X	X	X	X	X
Metabolism (e.g. cholesterol, LDL, HDL, insulin, triglycerides)	X	X	X	X	X
Metabolomics	X	X	X	X	X
DNA methylation	X	X	X	X	X
Transcriptomics (mRNA, miRNA)	X	X	X	X	X
Genome-wide association study (GWAS)	X	X	X	X	X

Maternal biological samples	---------- Timing of sampling ----------
	Pre-pregnancy	First trimester	Second trimester	Third trimester	Post pregnancy
Breast milk					X
Whole blood	X	X	X	X	X
Serum	X	X	X	X	X
Plasma	X	X	X	X	X
Buffy coat	X	X	X	X	X
Urine	X	X	X	X	X
Hair	X	X	X	X	X
Nails	X	X	X	X	X
Saliva	X	X	X	X	X
Purified DNA		X	X	X	X
Purified RNA		X	X	X	X
Red blood cells	X	X	X	X	X
Faeces	X	X	X	X	X

General information
	Pre-pregnancy	During pregnancy	<1	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18
Education	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Income	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Employment status	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Job title	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Single parenthood	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Home address	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Work address	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X

Paternal characteristics related to the index pregnancy/child
Fertility treatment	X
Age at child's birth	X
Ethnicity	X
Country of birth	X

Paternal exposures	---------- Timing of assessment ----------
	Pre-pregnancy	During pregnancy	Post pregnancy
Diet	X	X	X
Dietary supplements	X	X	X
Active smoking	X	X	X
Passive smoking	X	X	X
Alcohol consumption	X	X	X
Physical activity	X	X	X
Substance use	X	X	X
Medicine intake	X	X	X
Coffee drinking	X	X	X
Psychological distress (e.g. life-events, health behaviors, perceived stress)	X	X	X
Occupational exposures	X	X	X
Heavy lifts	X	X	X
Work hours	X	X	X
Air pollution	X	X	X
Access to green spaces	X	X	X
Built environment	X	X	X
Noise	X	X	X
Radiation, electromagnetic fields	X	X	X
Water contamination	X	X	X
Binge drinking	X	X	X
Pets	X	X	X
Farm animal exposure	X	X	X
Biomarkers of exposure
Mercury	X	X	X
Lead	X	X	X
Cadmium	X	X	X
Arsenic	X	X	X
Other metals	X	X	X
Polychlorinated biphenyl esters	X	X	X
Dicloro difenil tricloroetano	X	X	X
Hexachlorobenzene	X	X	X
Brominated flame retardants	X	X	X
Perflouralkyl compounds	X	X	X
Bisphenol A	X	X	X
Other bisphenols	X	X	X
Parabens	X	X	X
Benzophenones	X	X	X
Triclosan	X	X	X
Phthalates	X	X	X
Cotinine	X	X	X

Paternal health	---------- Timing of assessment ----------
	Pre-pregnancy	During pregnancy	Post pregnancy
Anthropometry
Weight	X	X	X
Height	X	X	X
Waist circumference	X	X	X
Clinical examinations
Blood pressure	X	X	X
Carotida intima media thickness	X	X	X
Pulse wave velocity	X	X	X
DEXA scans	X	X	X
Bio impedence	X	X	X
Mental and neurological development
Mental health	X	X	X
Cognitive function (e.g. intelligence quotient)	X	X	X
Respiratory health
Asthma	X	X	X
Allergy	X	X	X
Musculo‐skeletal disease
Back pain	X	X	X
Neck pain	X	X	X
Arthritis	X	X	X
Other health outcomes
Cancer	X	X	X
Diabetes	X	X	X
Cardio‐vascular disease	X	X	X
Family history of chronic disease	X	X	X
Infectious disease	X	X	X
Fever	X	X	X
Autoimmune disease	X	X	X
Urinary symptoms	X	X	X
Sexual problems	X	X	X
Sleep disturbance	X	X	X
Biomarkers of health
Nephrology (e.g. creatinine, albumin)	X	X	X
Thyroid hormones	X	X	X
Inflammatory markers (e.g. C-reactive protein cytokines)	X	X	X
Immunologic markers (e.g. immunoglobulines)	X	X	X
Cortisol	X	X	X
Vitamines and micronutrients	X	X	X
Gonadal axis hormones (e.g. testosterone, estradiol)	X	X	X
Metabolism (e.g. cholesterol, LDL, HDL, insulin, triglycerides)	X	X	X
Metabolomics	X	X	X
DNA methylation	X	X	X
Transcriptomics (mRNA, miRNA)	X	X	X
Genome-wide association study (GWAS)	X	X	X

Paternal biological samples	---------- Timing of sampling ----------
	Pre-pregnancy	During pregnancy	Post pregnancy
Semen	X	X	X
Whole blood	X	X	X
Serum	X	X	X
Plasma	X	X	X
Buffy coat	X	X	X
Urine	X	X	X
Hair	X	X	X
Nails	X	X	X
Saliva	X	X	X
Purified DNA		X
Purified RNA		X
Red blood cells	X	X	X
Faeces	X	X	X

Comments and ideas

Comments and ideas