Identification

• Cohort, name
  Generation XXI
  
  Cohort, abbreviation
  G21
  
  Cohort, country
  Portugal
  
  Cohort, website
  http://www.geracao21.com
  
  Key reference
  Alves E, Correia S, Barros H, Azevedo A. Prevalence of self-reported cardiovascular risk factors in Portuguese women: a survey after delivery. Int J Public Health. 2012. Oct;57(5):837-47
  
  Date of filling in this questionnaire:
  2013-05-29
  
  
• Principal investigator(s), name
  Henrique Barros
  
  Principal investigator(s), e-mail address
  hbarros@med.up.pt
  
  Principal investigator(s), institution
  Department of Clinical Epidemiology, Predictive Medicine and Public Health, University of Porto Medical School, Porto, Portug
  
  Contact person(s), name
  Ana Cristina Santos
  
  Contact person(s), e-mail address
  acsantos@med.up.pt
  
  Contact person(s), institution address
  Al Prof Hernani Monteiro 4200-319 Porto
  
  

Basic description

• Main aim of cohort
  Although this is a multi-purpose cohort on child health and development, its two main specific aims are: •To study determinants of differences in foetal and early life growth and body composition. •To relate these changes to obesity and other health outcomes later in life.
  
  Recruited number of:
  8647 children / 8495 mothers / 4351 fathers / 0 grand parents / 0 other family members
  
  Approximate proportion of source population included
  70
  
  Source population
  Region-based
  
  
• Period of enrollment
  Start: 2005-04-10, End: 2006-08-31
  
  Enrollment period
  At birth  to
  
  Enrollment criteria, please specify
  Main group - at birth: Gestational age >23 weeks; birth at one of the 5 level III public maternities from Porto Metropolitan Area; maternal residence at one of the six municipalities from this region. Sub-group - during pregnancy: 1st antenatal visit up to the 13th gestational week in two (of the five) maternity units.
  
  Planned age of children at end of follow-up
  

Questionnaire or registry data and biological samples

• Children
• Mothers
• Fathers

General information	---------- Child age (years) at assessment ----------
	<1	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	>18
Home address
School address

Birth outcomes
Birth weight	X
Birth length	X
Gestational age at birth	X
Apgar score	X
Congenital malformations	X
Stillbirth (>= 22 weeks)
Child’s sex	X
Head circumference
Biparietal diameter

Child exposures	---------- Child age (years) at assessment ----------
	<1	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	>18
Breast feeding	X	X	X
Diet	X	X	X		X			X
Dietary supplements
Childcare attendance	X	X	X		X
Active smoking
Passive smoking	X	X	X		X			X
Alcohol consumption
Physical activity					X			X
Substance use
Medicine intake	X	X	X		X			X
Vaccinations	X	X	X		X			X
Psychological distress (e.g. bullying)
Air pollution
Access to green spaces
Built environment
Noise
Radiation, electromagnetic fields
Water contamination
Pets
Farm animal exposure
Biomarkers of exposure
Mercury
Lead
Cadmium
Arsenic
Other metals
Polychlorinated biphenyl esters
Dicloro difenil tricloroetano
Hexachlorobenzene
Brominated flame retardants
Perflouralkyl compounds
Bisphenol A
Other bisphenols
Parabens
Benzophenones
Triclosan
Phthalates
Cotinine

Child development and health	---------- Child age (years) at assessment ----------
	<1	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	>18
Anthropometry
Weight	X	X	X		X			X
Height	X	X	X		X			X
Head circumference	X	X	X		X
Abdominal circumference
Arm circumference
Wrist circumference
Waist circumference
Clinical examinations
Blood pressure					X			X
Carotida intima media thickness
Pulse wave velocity
Cardiac ultrasound
Renal ultrasound
Brain MRI
Other MRI
DEXA‐scan results								X
Skin folds
Bio impedence
Oscilometry
Spirometry
Skin prick test
Bronchial challenge test
Reversibility test
Dental observations
Mental and neurological development
Cognitive function
School performance
Language
ADHD
Autism
Sleep disturbances	X		X		X			X
Mental health
Respiratory health
Asthma
Allergy
Allergic rhinitis
Eczema
Respiratory tract infections
Senses
Vision
Hearing
Musculo-skeletal health
Back pain
Neck pain
Growing pains
Sexual maturation
Tanner stage
Pubertal development stage
Age at menarche
Age at voice change
2D/4D digit ratio
Nocturnal ejaculation
Acne
Other health outcomes
Cancer
Diabetes								X
Congenital anomalies					X			X
Biomarkers of health
Nephrology (e.g. creatinine, albumin)
Thyroid hormones
Inflammatory markers (e.g. C-reactive protein cytokines)
Immunologic markers (e.g. immunoglobulines)
Cortisol
Vitamines and micronutrients
Gonadal axis hormones (e.g. testosterone, estradiol)
Metabolism (e.g. cholesterol, LDL, HDL, insulin, triglycerides)
Metabolomics
DNA methylation
Transcriptomics (mRNA, miRNA)
Genome-wide association study (GWAS)

Child biological samples	---------- Child age (years) at sampling ----------
	At birth	0	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	>18
Whole blood
Serum						X			X
Plasma
Buffy coat
Urine
Hair
Teeth									X
Nails
Saliva
Purified DNA
Purified RNA
Red blood cells
Umbilical cord blood/tissue	X
Blood smear
Faeces/meconium
Exhaled breathe condensate
Sputum induction
Placenta

General information
	Pre-pregnancy	During pregnancy	<1	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18
Education
Income
Employment status
Job title
Single parenthood
Home address
Work address

Maternal characteristics related to the index pregnancy/child
Age at birth	X
Ethnicity
Country of birth
Planned pregnancy	X
Time to pregnancy	X
Fertility treatment	X
Prior spontaneous abortion (<22w)	X
Prior births	X
Ultrasound measurements
Doppler measurements
Preeclampsia	X
Gestational diabetes
Gestational hypertension
Gestational duration	X
Mode of delivery	X
Prenatal diagnostics	X
Medical termination of pregnancy	X

Maternal Exposures	---------- Timing of assessment ----------
	Pre-pregnancy	First trimester	Second trimester	Third trimester	Post pregnancy
Diet		X	X	X	X
Dietary supplements		X	X	X	X
Active smoking		X	X	X	X
Passive smoking		X	X	X	X
Alcohol consumption		X	X	X	X
Physical activity		X	X	X
Substance use		X	X	X	X
Medicine intake		X	X	X	X
Coffee drinking		X	X	X	X
Psychological distress (e.g. life-events, health behaviors, perceived stress)		X	X	X	X
Occupational exposures
Heavy lifts
Work hours
Air pollution
Access to green spaces
Built environment
Noise
Radiation, electromagnetic fields
Water contamination
Binge drinking		X	X	X	X
Pets
Farm animal exposure
Biomarkers of exposure
Mercury
Lead
Cadmium
Arsenic
Other metals
Polychlorinated biphenyl esters
Dicloro difenil tricloroetano
Hexachlorobenzene
Brominated flame retardants
Perflouralkyl compounds
Bisphenol A
Other bisphenols
Parabens
Benzophenones
Triclosan
Phthalates
Cotinine

Maternal health	---------- Timing of assessment ----------
	Pre-pregnancy	First trimester	Second trimester	Third trimester	Post pregnancy
Anthropometry
Weight		X			X
Height					X
Waist circumference
Clinical examinations
Blood pressure					X
Carotida intima media thickness
Pulse wave velocity
DEXA scans
Bioimpedence
Mental and neurological development
Mental health					X
Cognitive function (e.g. intelligence quotient)
Respiratory health
Asthma
Allergy
Musculo‐skeletal disease					X
Back pain
Neck pain
Arthritis
Other health outcomes
Cancer					X
Diabetes					X
Cardio‐vascular disease					X
Family history of chronic disease
Infectious disease
Fever
Autoimmune disease					X
Urinary symptoms
Sexual problems
Sleep disturbance
Biomarkers of health
Nephrology (e.g. creatinine, albumin)
Thyroid hormones
Inflammatory markers (e.g. C-reactive protein cytokines)
Immunologic markers (e.g. immunoglobulines)
Cortisol
Vitamines and micronutrients
Gonadal axis hormones (e.g. testosterone, estradiol)
Metabolism (e.g. cholesterol, LDL, HDL, insulin, triglycerides)
Metabolomics
DNA methylation
Transcriptomics (mRNA, miRNA)
Genome-wide association study (GWAS)

Maternal biological samples	---------- Timing of sampling ----------
	Pre-pregnancy	First trimester	Second trimester	Third trimester	Post pregnancy
Breast milk
Whole blood					X
Serum					X
Plasma
Buffy coat
Urine
Hair
Nails
Saliva
Purified DNA
Purified RNA
Red blood cells
Faeces

General information
	Pre-pregnancy	During pregnancy	<1	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18
Education
Income
Employment status
Job title
Single parenthood
Home address
Work address

Paternal characteristics related to the index pregnancy/child
Fertility treatment
Age at child's birth	X
Ethnicity
Country of birth

Paternal exposures	---------- Timing of assessment ----------
	Pre-pregnancy	During pregnancy	Post pregnancy
Diet
Dietary supplements
Active smoking			X
Passive smoking			X
Alcohol consumption			X
Physical activity
Substance use			X
Medicine intake
Coffee drinking
Psychological distress (e.g. life-events, health behaviors, perceived stress)
Occupational exposures			X
Heavy lifts
Work hours
Air pollution
Access to green spaces
Built environment
Noise
Radiation, electromagnetic fields
Water contamination
Binge drinking
Pets
Farm animal exposure
Biomarkers of exposure
Mercury
Lead
Cadmium
Arsenic
Other metals
Polychlorinated biphenyl esters
Dicloro difenil tricloroetano
Hexachlorobenzene
Brominated flame retardants
Perflouralkyl compounds
Bisphenol A
Other bisphenols
Parabens
Benzophenones
Triclosan
Phthalates
Cotinine

Paternal health	---------- Timing of assessment ----------
	Pre-pregnancy	During pregnancy	Post pregnancy
Anthropometry
Weight			X
Height			X
Waist circumference
Clinical examinations
Blood pressure			X
Carotida intima media thickness
Pulse wave velocity
DEXA scans
Bio impedence
Mental and neurological development
Mental health			X
Cognitive function (e.g. intelligence quotient)
Respiratory health
Asthma
Allergy
Musculo‐skeletal disease			X
Back pain
Neck pain
Arthritis
Other health outcomes
Cancer			X
Diabetes			X
Cardio‐vascular disease			X
Family history of chronic disease
Infectious disease
Fever
Autoimmune disease			X
Urinary symptoms
Sexual problems
Sleep disturbance
Biomarkers of health
Nephrology (e.g. creatinine, albumin)
Thyroid hormones
Inflammatory markers (e.g. C-reactive protein cytokines)
Immunologic markers (e.g. immunoglobulines)
Cortisol
Vitamines and micronutrients
Gonadal axis hormones (e.g. testosterone, estradiol)
Metabolism (e.g. cholesterol, LDL, HDL, insulin, triglycerides)
Metabolomics
DNA methylation
Transcriptomics (mRNA, miRNA)
Genome-wide association study (GWAS)

Paternal biological samples	---------- Timing of sampling ----------
	Pre-pregnancy	During pregnancy	Post pregnancy
Semen
Whole blood			X
Serum			X
Plasma
Buffy coat
Urine
Hair
Nails
Saliva
Purified DNA
Purified RNA
Red blood cells
Faeces

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