• Cohort, name
    Multiple Births Cohort Study

    Cohort, abbreviation
    MUBICOS

    Cohort, country
    Italy (Italia)

    Key reference
    Brescianini S, Cotichini R, Serino L, Medda E. Studio longitudinale su una coorte di neonati gemelli. In: Salemi M, Penna L, DIppolito C, ed. Convegno I gemelli in età pediatrica: epidemiologia, clinica e psicologia. Istituto Superiore di Sanità. Roma, 16 novembre 2009. Atti. Roma: Istituto Superiore di Sanità; 2010. (Rapporti ISTISAN 10/07). p.56-58.

    Date of filling in this questionnaire:
    2011-10-14

  • Principal investigator(s), name
    Sonia Brescianini

    Principal investigator(s), e-mail address
    sonia.brescianini@iss.it

    Principal investigator(s), institution
    Istituto Superiore di Sanità, Rome, Italy

    Contact person(s), name
    Sonia Brescianini

    Contact person(s), e-mail address
    SONIA.BRESCIANINI@ISS.IT

    Contact person(s), institution address
    v.le Regina Elena 299, 00161, Rome, Italy

  • Main aim of cohort
    The Italian Twin Registry (ITR) has started the enrolment of the cohort of newborn twins/multiples with the goal of following them during their childhood. This longitudinal cohort will allow studies on heritability and gene-environment interaction for major neonatal and paediatric outcomes, as well as etiological studies on the entire cohort given the extensive information on prenatal and neonatal life. The project is collecting saliva samples for DNA extraction from parents and twins/multiples. Main exposures are: diet, drugs, occupational exposures, physical activity for both parents during pregnancy; immunizations, infections, diet, environmental exposures for the offspring during the first years of life. Main outcomes are: growth, developmental milestones, allergies and sleeping behaviour.

    Recruited number of:
    1000 children / 500 mothers / 500 fathers / 0 grand parents / 0 other family members

    Approximate proportion of source population included
    50

    Source population
    Other, please describe8 italian hospitals

  • Period of enrollment
    Start: 2009-01-01, End: 0000-01-01

    Enrollment period
    At birth  to

    Enrollment criteria, please specify
    Twins must be both liveborn and at least 32 weeks of gestation at least 1 parent has to understand and speak italian

    Exclusion criteria, please specify
    one of the tiwns is transferred to antoher hospital shortly after birth or dies shortly after birth

    Planned age of children at end of follow-up
General information ---------- Child age (years) at assessment ----------
<1 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18
Home address
School address
Birth outcomes
Birth weightX
Birth lengthX
Gestational age at birthX
Apgar scoreX
Congenital malformationsX
Stillbirth (>= 22 weeks)
Child’s sex
Head circumference
Biparietal diameter
Child exposures ---------- Child age (years) at assessment ----------
<1 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18
Breast feedingXX
DietXXXXXX
Dietary supplements
Childcare attendanceXXXXXX
Active smoking
Passive smokingXXXXX
Alcohol consumption
Physical activityXXX
Substance use
Medicine intakeXXXXXX
VaccinationsXXXXXX
Psychological distress (e.g. bullying)
Air pollution
Access to green spaces
Built environment
Noise
Radiation, electromagnetic fields
Water contamination
Pets
Farm animal exposure
Biomarkers of exposure
Mercury
Lead
Cadmium
Arsenic
Other metals
Polychlorinated biphenyl esters
Dicloro difenil tricloroetano
Hexachlorobenzene
Brominated flame retardants
Perflouralkyl compounds
Bisphenol A
Other bisphenols
Parabens
Benzophenones
Triclosan
Phthalates
Cotinine
Child development and health ---------- Child age (years) at assessment ----------
<1 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18
Anthropometry
WeightXXXXXX
HeightXXXXXX
Head circumferenceXXX
Abdominal circumference
Arm circumference
Wrist circumference
Waist circumference
Clinical examinations
Blood pressure
Carotida intima media thickness
Pulse wave velocity
Cardiac ultrasound
Renal ultrasound
Brain MRI
Other MRI
DEXA‐scan results
Skin folds
Bio impedence
Oscilometry
Spirometry
Skin prick test
Bronchial challenge test
Reversibility test
Dental observations
Mental and neurological development
Cognitive functionX
School performanceX
LanguageXX
ADHD
Autism
Sleep disturbancesXXXX
Mental health
Respiratory health
Asthma
Allergy
Allergic rhinitis
Eczema
Respiratory tract infections
Senses
Vision
Hearing
Musculo-skeletal health
Back pain
Neck pain
Growing pains
Sexual maturation
Tanner stage
Pubertal development stage
Age at menarche
Age at voice change
2D/4D digit ratio
Nocturnal ejaculation
Acne
Other health outcomes
Cancer
Diabetes
Congenital anomaliesX
Biomarkers of health
Nephrology (e.g. creatinine, albumin)
Thyroid hormones
Inflammatory markers (e.g. C-reactive protein cytokines)
Immunologic markers (e.g. immunoglobulines)
Cortisol
Vitamines and micronutrients
Gonadal axis hormones (e.g. testosterone, estradiol)
Metabolism (e.g. cholesterol, LDL, HDL, insulin, triglycerides)
Metabolomics
DNA methylation
Transcriptomics (mRNA, miRNA)
Genome-wide association study (GWAS)
Child biological samples ---------- Child age (years) at sampling ----------
At birth 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18
Whole blood
Serum
Plasma
Buffy coat
Urine
Hair
Teeth
Nails
SalivaX
Purified DNA
Purified RNA
Red blood cells
Umbilical cord blood/tissue
Blood smear
Faeces/meconium
Exhaled breathe condensate
Sputum induction
Placenta
General information
Pre-pregnancy During pregnancy <1 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18
Education
Income
Employment status
Job title
Single parenthood
Home address
Work address
Maternal characteristics related to the index pregnancy/child
Age at birthX
EthnicityX
Country of birth
Planned pregnancyX
Time to pregnancy
Fertility treatmentX
Prior spontaneous abortion (<22w)X
Prior birthsX
Ultrasound measurements
Doppler measurements
PreeclampsiaX
Gestational diabetes
Gestational hypertension
Gestational durationX
Mode of deliveryX
Prenatal diagnosticsX
Medical termination of pregnancyX
Maternal Exposures ---------- Timing of assessment ----------
Pre-pregnancy First trimester Second trimester Third trimester Post pregnancy
Diet
Dietary supplementsXXXXX
Active smokingXXXXX
Passive smokingXXXXX
Alcohol consumptionXXXXX
Physical activity
Substance use
Medicine intakeXXXXX
Coffee drinkingXXXXX
Psychological distress (e.g. life-events, health behaviors, perceived stress)
Occupational exposuresXXXXX
Heavy lifts
Work hours
Air pollution
Access to green spaces
Built environment
Noise
Radiation, electromagnetic fields
Water contamination
Binge drinking
Pets
Farm animal exposure
Biomarkers of exposure
Mercury
Lead
Cadmium
Arsenic
Other metals
Polychlorinated biphenyl esters
Dicloro difenil tricloroetano
Hexachlorobenzene
Brominated flame retardants
Perflouralkyl compounds
Bisphenol A
Other bisphenols
Parabens
Benzophenones
Triclosan
Phthalates
Cotinine
Maternal health ---------- Timing of assessment ----------
Pre-pregnancy First trimester Second trimester Third trimester Post pregnancy
Anthropometry
WeightXX
HeightXX
Waist circumference
Clinical examinations
Blood pressure
Carotida intima media thickness
Pulse wave velocity
DEXA scans
Bioimpedence
Mental and neurological development
Mental healthXXX
Cognitive function (e.g. intelligence quotient)
Respiratory health
Asthma
Allergy
Musculo‐skeletal diseaseXXX
Back pain
Neck pain
Arthritis
Other health outcomes
CancerXXX
DiabetesXXX
Cardio‐vascular diseaseXXX
Family history of chronic disease
Infectious diseaseXXX
Fever
Autoimmune diseaseXXX
Urinary symptoms
Sexual problems
Sleep disturbance
Biomarkers of health
Nephrology (e.g. creatinine, albumin)
Thyroid hormones
Inflammatory markers (e.g. C-reactive protein cytokines)
Immunologic markers (e.g. immunoglobulines)
Cortisol
Vitamines and micronutrients
Gonadal axis hormones (e.g. testosterone, estradiol)
Metabolism (e.g. cholesterol, LDL, HDL, insulin, triglycerides)
Metabolomics
DNA methylation
Transcriptomics (mRNA, miRNA)
Genome-wide association study (GWAS)
Maternal biological samples ---------- Timing of sampling ----------
Pre-pregnancy First trimester Second trimester Third trimester Post pregnancy
Breast milk
Whole blood
Serum
Plasma
Buffy coat
Urine
Hair
Nails
SalivaX
Purified DNA
Purified RNA
Red blood cells
Faeces
General information
Pre-pregnancy During pregnancy <1 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18
Education
Income
Employment status
Job title
Single parenthood
Home address
Work address
Paternal characteristics related to the index pregnancy/child
Fertility treatment
Age at child's birthX
EthnicityX
Country of birth
Paternal exposures ---------- Timing of assessment ----------
Pre-pregnancy During pregnancy Post pregnancy
Diet
Dietary supplements
Active smokingXXX
Passive smokingXXX
Alcohol consumptionXXX
Physical activity
Substance use
Medicine intake
Coffee drinking
Psychological distress (e.g. life-events, health behaviors, perceived stress)
Occupational exposures
Heavy lifts
Work hours
Air pollution
Access to green spaces
Built environment
Noise
Radiation, electromagnetic fields
Water contamination
Binge drinking
Pets
Farm animal exposure
Biomarkers of exposure
Mercury
Lead
Cadmium
Arsenic
Other metals
Polychlorinated biphenyl esters
Dicloro difenil tricloroetano
Hexachlorobenzene
Brominated flame retardants
Perflouralkyl compounds
Bisphenol A
Other bisphenols
Parabens
Benzophenones
Triclosan
Phthalates
Cotinine
Paternal health ---------- Timing of assessment ----------
Pre-pregnancy During pregnancy Post pregnancy
Anthropometry
Weight
Height
Waist circumference
Clinical examinations
Blood pressure
Carotida intima media thickness
Pulse wave velocity
DEXA scans
Bio impedence
Mental and neurological development
Mental healthXXX
Cognitive function (e.g. intelligence quotient)
Respiratory health
Asthma
Allergy
Musculo‐skeletal diseaseXXX
Back pain
Neck pain
Arthritis
Other health outcomes
CancerXXX
DiabetesXXX
Cardio‐vascular diseaseXXX
Family history of chronic disease
Infectious diseaseXXX
Fever
Autoimmune diseaseXXX
Urinary symptoms
Sexual problems
Sleep disturbance
Biomarkers of health
Nephrology (e.g. creatinine, albumin)
Thyroid hormones
Inflammatory markers (e.g. C-reactive protein cytokines)
Immunologic markers (e.g. immunoglobulines)
Cortisol
Vitamines and micronutrients
Gonadal axis hormones (e.g. testosterone, estradiol)
Metabolism (e.g. cholesterol, LDL, HDL, insulin, triglycerides)
Metabolomics
DNA methylation
Transcriptomics (mRNA, miRNA)
Genome-wide association study (GWAS)
Paternal biological samples ---------- Timing of sampling ----------
Pre-pregnancy During pregnancy Post pregnancy
Semen
Whole blood
Serum
Plasma
Buffy coat
Urine
Hair
Nails
SalivaX
Purified DNA
Purified RNA
Red blood cells
Faeces
Comments and ideas
Exposures for parents are mostly assessed after birth or at first contact in pregnancy but they refere to different periods: 12 months before pregnancy, 9 mothns of pregnancy and and the children follow-up. Expsosure of children especially after 2 years of age are still being established so they are subject to change.