• Cohort, name
    PRINCE

    Cohort, abbreviation
    Prenatal Identification of Children's Health

    Cohort, country
    Germany (Deutschland)

    Cohort, website
    www.uke.de/prince

    Date of filling in this questionnaire:
    2015-03-31

  • Principal investigator(s), name
    Petra Arck & Anke Diemert

    Principal investigator(s), e-mail address
    p.arck@uke.de

    Principal investigator(s), institution
    University Medical Center Hamburg-Eppendorf

    Contact person(s), name
    Petra Arck, Anke Diemert

    Contact person(s), e-mail address
    p.arck@uke.de, a.diemert@uke.de

    Contact person(s), institution address
    Department of Obstetrics and Fetal Medicine, University Medical Center Hamburg

  • Main aim of cohort
    Children’s health is critically dependent of the prenatal well-being of the mother, and a number of observational studies have identified an impaired immunity in children upon prenatal challenges, such as stress perception, medication, nutrition or infection. However, concerted and in-depth studies assessing how such prenatal challenges may alter foetal development and subsequently children’s health in humans are largely lacking. In the PRINCE study, we address this limitation by recruiting healthy pregnant women into a cohort study, followed up by the assessment of children’s health and immunity at birth and during the first years of life. Prenatal challenges and postnatal confounders are thoroughly documented, along with the collection of biological samples. This unique cohort now allows for testing the impact of prenatal challenges on children’s health, and provides the basis for translating findings arising from functional and reductionist approaches using mouse models.

    Recruited number of:
    750 children / 750 mothers / 0 fathers / 0 grand parents / 0 other family members

    Approximate proportion of source population included
    0

    Source population
    Region-based

  • Period of enrollment
    Start: 2012-07-01, End: 0000-00-00

    Enrollment period
    Pregnancy, week 14 to 36

    Enrollment criteria, please specify
    maternal age of at least 18 years singleton pregnancy ability to give informed consent

    Exclusion criteria, please specify
    pregnancies resulting from assisted reproductive technologies

    Planned age of children at end of follow-up
    Life-long / unrestricted
General information ---------- Child age (years) at assessment ----------
<1 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18
Home addressXXXXXXX
School address
Birth outcomes
Birth weightX
Birth lengthX
Gestational age at birthX
Apgar scoreX
Congenital malformationsX
Stillbirth (>= 22 weeks)X
Child’s sexX
Head circumference
Biparietal diameter
Child exposures ---------- Child age (years) at assessment ----------
<1 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18
Breast feedingXXXX
Diet
Dietary supplements
Childcare attendanceXXXXXXX
Active smoking
Passive smokingXXXXXXX
Alcohol consumption
Physical activityX
Substance use
Medicine intakeXXXXXX
VaccinationsXXXXXX
Psychological distress (e.g. bullying)
Air pollution
Access to green spaces
Built environment
Noise
Radiation, electromagnetic fields
Water contamination
PetsXXXXXX
Farm animal exposure
Biomarkers of exposure
Mercury
Lead
Cadmium
Arsenic
Other metals
Polychlorinated biphenyl esters
Dicloro difenil tricloroetano
Hexachlorobenzene
Brominated flame retardants
Perflouralkyl compounds
Bisphenol A
Other bisphenols
Parabens
Benzophenones
Triclosan
Phthalates
Cotinine
Child development and health ---------- Child age (years) at assessment ----------
<1 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18
Anthropometry
WeightXXXXXX
HeightXXXXXX
Head circumferenceX
Abdominal circumferenceX
Arm circumferenceX
Wrist circumferenceX
Waist circumferenceX
Clinical examinations
Blood pressureX
Carotida intima media thickness
Pulse wave velocity
Cardiac ultrasound
Renal ultrasound
Brain MRI
Other MRI
DEXA‐scan results
Skin foldsX
Bio impedence
Oscilometry
SpirometryX
Skin prick test
Bronchial challenge test
Reversibility test
Dental observationsX
Mental and neurological development
Cognitive functionX
School performance
Language
ADHD
Autism
Sleep disturbances
Mental health
Respiratory health
AsthmaX
AllergyX
Allergic rhinitisX
EczemaX
Respiratory tract infectionsXXXXXX
Senses
Vision
Hearing
Musculo-skeletal health
Back painX
Neck painX
Growing painsX
Sexual maturation
Tanner stage
Pubertal development stage
Age at menarche
Age at voice change
2D/4D digit ratio
Nocturnal ejaculation
Acne
Other health outcomes
CancerX
DiabetesX
Congenital anomaliesXX
Biomarkers of health
Nephrology (e.g. creatinine, albumin)
Thyroid hormones
Inflammatory markers (e.g. C-reactive protein cytokines)X
Immunologic markers (e.g. immunoglobulines)X
Cortisol
Vitamines and micronutrientsX
Gonadal axis hormones (e.g. testosterone, estradiol)
Metabolism (e.g. cholesterol, LDL, HDL, insulin, triglycerides)
Metabolomics
DNA methylation
Transcriptomics (mRNA, miRNA)
Genome-wide association study (GWAS)
Child biological samples ---------- Child age (years) at sampling ----------
At birth 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18
Whole bloodX
SerumX
PlasmaX
Buffy coat
Urine
Hair
Teeth
Nails
Saliva
Purified DNA
Purified RNA
Red blood cells
Umbilical cord blood/tissue
Blood smear
Faeces/meconium
Exhaled breathe condensate
Sputum induction
Placenta
General information
Pre-pregnancy During pregnancy <1 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18
Education
Income
Employment status
Job title
Single parenthood
Home address
Work address
Maternal characteristics related to the index pregnancy/child
Age at birth
Ethnicity
Country of birth
Planned pregnancy
Time to pregnancy
Fertility treatment
Prior spontaneous abortion (<22w)
Prior births
Ultrasound measurements
Doppler measurements
Preeclampsia
Gestational diabetes
Gestational hypertension
Gestational duration
Mode of delivery
Prenatal diagnostics
Medical termination of pregnancy
Maternal Exposures ---------- Timing of assessment ----------
Pre-pregnancy First trimester Second trimester Third trimester Post pregnancy
Diet
Dietary supplements
Active smoking
Passive smoking
Alcohol consumption
Physical activity
Substance use
Medicine intake
Coffee drinking
Psychological distress (e.g. life-events, health behaviors, perceived stress)
Occupational exposures
Heavy lifts
Work hours
Air pollution
Access to green spaces
Built environment
Noise
Radiation, electromagnetic fields
Water contamination
Binge drinking
Pets
Farm animal exposure
Biomarkers of exposure
Mercury
Lead
Cadmium
Arsenic
Other metals
Polychlorinated biphenyl esters
Dicloro difenil tricloroetano
Hexachlorobenzene
Brominated flame retardants
Perflouralkyl compounds
Bisphenol A
Other bisphenols
Parabens
Benzophenones
Triclosan
Phthalates
Cotinine
Maternal health ---------- Timing of assessment ----------
Pre-pregnancy First trimester Second trimester Third trimester Post pregnancy
Anthropometry
Weight
Height
Waist circumference
Clinical examinations
Blood pressure
Carotida intima media thickness
Pulse wave velocity
DEXA scans
Bioimpedence
Mental and neurological development
Mental health
Cognitive function (e.g. intelligence quotient)
Respiratory health
Asthma
Allergy
Musculo‐skeletal disease
Back pain
Neck pain
Arthritis
Other health outcomes
Cancer
Diabetes
Cardio‐vascular disease
Family history of chronic disease
Infectious disease
Fever
Autoimmune disease
Urinary symptoms
Sexual problems
Sleep disturbance
Biomarkers of health
Nephrology (e.g. creatinine, albumin)
Thyroid hormones
Inflammatory markers (e.g. C-reactive protein cytokines)
Immunologic markers (e.g. immunoglobulines)
Cortisol
Vitamines and micronutrients
Gonadal axis hormones (e.g. testosterone, estradiol)
Metabolism (e.g. cholesterol, LDL, HDL, insulin, triglycerides)
Metabolomics
DNA methylation
Transcriptomics (mRNA, miRNA)
Genome-wide association study (GWAS)
Maternal biological samples ---------- Timing of sampling ----------
Pre-pregnancy First trimester Second trimester Third trimester Post pregnancy
Breast milk
Whole blood
Serum
Plasma
Buffy coat
Urine
Hair
Nails
Saliva
Purified DNA
Purified RNA
Red blood cells
Faeces
General information
Pre-pregnancy During pregnancy <1 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18
Education
Income
Employment status
Job title
Single parenthood
Home address
Work address
Paternal characteristics related to the index pregnancy/child
Fertility treatment
Age at child's birth
Ethnicity
Country of birth
Paternal exposures ---------- Timing of assessment ----------
Pre-pregnancy During pregnancy Post pregnancy
Diet
Dietary supplements
Active smoking
Passive smoking
Alcohol consumption
Physical activity
Substance use
Medicine intake
Coffee drinking
Psychological distress (e.g. life-events, health behaviors, perceived stress)
Occupational exposures
Heavy lifts
Work hours
Air pollution
Access to green spaces
Built environment
Noise
Radiation, electromagnetic fields
Water contamination
Binge drinking
Pets
Farm animal exposure
Biomarkers of exposure
Mercury
Lead
Cadmium
Arsenic
Other metals
Polychlorinated biphenyl esters
Dicloro difenil tricloroetano
Hexachlorobenzene
Brominated flame retardants
Perflouralkyl compounds
Bisphenol A
Other bisphenols
Parabens
Benzophenones
Triclosan
Phthalates
Cotinine
Paternal health ---------- Timing of assessment ----------
Pre-pregnancy During pregnancy Post pregnancy
Anthropometry
Weight
Height
Waist circumference
Clinical examinations
Blood pressure
Carotida intima media thickness
Pulse wave velocity
DEXA scans
Bio impedence
Mental and neurological development
Mental health
Cognitive function (e.g. intelligence quotient)
Respiratory health
Asthma
Allergy
Musculo‐skeletal disease
Back pain
Neck pain
Arthritis
Other health outcomes
Cancer
Diabetes
Cardio‐vascular disease
Family history of chronic disease
Infectious disease
Fever
Autoimmune disease
Urinary symptoms
Sexual problems
Sleep disturbance
Biomarkers of health
Nephrology (e.g. creatinine, albumin)
Thyroid hormones
Inflammatory markers (e.g. C-reactive protein cytokines)
Immunologic markers (e.g. immunoglobulines)
Cortisol
Vitamines and micronutrients
Gonadal axis hormones (e.g. testosterone, estradiol)
Metabolism (e.g. cholesterol, LDL, HDL, insulin, triglycerides)
Metabolomics
DNA methylation
Transcriptomics (mRNA, miRNA)
Genome-wide association study (GWAS)
Paternal biological samples ---------- Timing of sampling ----------
Pre-pregnancy During pregnancy Post pregnancy
Semen
Whole blood
Serum
Plasma
Buffy coat
Urine
Hair
Nails
Saliva
Purified DNA
Purified RNA
Red blood cells
Faeces
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